Monitoring the spotted salamander by environmental dna (edna), a conservation challenge

16 November 2023

Salamandra salamandra

The Spotted salamander Salamandra salamandra is a well-known amphibian in our regions, particularly for its distinctive colours. Fully protected, the species particularly appreciates deciduous forest valleys with high ambient humidity, typically due to the presence of streams, springs, or seeps that are essential for its reproduction.

Individuals are mainly active at night in humid weather and can be observed throughout the year. An uncommon phenomenon among amphibians in our regions, the spotted salamander is strictly terrestrial, with females only approaching water to deposit their larvae.

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Detection through Environmental DNA

Complementary to traditional inventories, environmental DNA (eDNA) inventory methods are proving highly promising for enhancing the monitoring and detection of species like the Spotted salamander.

E-BIOM has recently developed and validated an eDNA detection method. By sampling water within the biotopes likely to host the species, it is possible to collect traces of DNA (such as mucus) left by the larvae and adults in the vicinity. This DNA is then analysed in the laboratory to confirm or deny the presence of the Spotted salamander on the sampled site.

The interest of the method?

  • Increasing the detectability of this discreet and nocturnal species.
  • Clarifying its distribution within a territory.
  • Guiding conservation and restoration measures.
  • Etc.

Salamander Chytridiomycosis

In 2013, Batrachochytrium salamandrivorans (Bsal), a pathogenic fungus originating from Asia, was detected for the first time in Belgium. This threat is real, as in the Netherlands, this pathogen, also known as chytridiomycosis, caused the extinction of 96% of the salamander populations in just three years…

A national action plan was then established to limit the spread of Bsal on the territory. In Wallonia, a few cases of contamination a few cases of contamination have been discovered but seem to be localized.

Today, Belgian populations of the Spotted salamander seem stable, but precautionary measures are still required to prevent the spread of this highly virulent pathogen.

 

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Research & Development

Having validated the detection of the Spotted salamander through eDNA, E-BIOM now seeks to develop a method for detecting Batrachochytrium salamandrivorans by eDNA to enhance monitoring and halt its spread once Bsal has been detected. Environmental epidemiological monitoring is part of E-BIOM’s expertise, and our team of scientists is dedicated to contributing daily to environmental preservation.

E-BIOM, Your Biodiversity Partner

Combining an analysis laboratory with a scientific consulting office, E-BIOM offers numerous services dedicated to biodiversity preservation. We work closely with both companies and public stakeholders to implement new systems that will rethink our relationship with Nature and its living beings.

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